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KMID : 0525720180230040277
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Journal of Chitin and Chitosan
2018 Volume.23 No. 4 p.277 ~ p.284
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Anti-allergic Effect of Eckolona cava Ethyl Acetate Fraction of on IgE/BSA-stimulated Bone Marrow-derived Cultured Mast Cells
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Han Eui-Jeong
Kim Hyun-Soo
Shin Eun-Ji
Kim Min-Ju
Han Hee-Jin
Jeon You-Jin
Jee Young-Heun
Ahn Gin-Nae
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Abstract
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In this study, we investigated the anti-allergic effect of the ethyl acetate fraction of Ecklonia cava (EC-EtoAc) on the immunoglobulin E (IgE)/bovine serum albumin (BSA)-mediated activation of bone marrow-derived cultured mast cells (BMCMCs). We revealed that the 62.5 μg/ml of EC-fractions (EC-CHCl3, EC-Hexane and EC-EtoAc) inhibited IgE/BSAactivated β-hexosaminidase release from BMCMCs without cytotoxicity. Especially, EC-EtoAc showed the higher β- hexosaminidase release than the others. Also, EC-EtoAc reduced the expression levels of cytokines such as interleukin (IL)-1β, IL-4, IL-5, IL-6, IL-10, IL-13, interferon (IFN)-γ and tumor necrosis factor (TNF)-α and a chemokine, thymus- and activationregulated chemokine (TARC), compared to the only IgE/BSA-treated BMCMCs. Furthermore, EC-EtoAc significantly prevented the binding of IgE to Fc epsilon receptor (FcεR)I and reduced the FcεRI expression on the sensitized BMCMCs. Taken together, these results suggest that E. cava may be the natural agent with beneficial potentials for the treatment of type I allergic diseases induced by mast cell activation.
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KEYWORD
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Ecklonia cava, Ethyl acetate fraction, Anti-allergic effect, BMCMCs
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